Search results for "Réponse au stress"
showing 2 items of 2 documents
Study of molecular mechanisms of stress response in Oenococcus oeni and implementation of tools for the functional exploration of enological genes
2015
O. oeni is responsible for wine malolactic fermentation. As any organism, O. oeni tries to adapt its physiology to environmental fluctuations by producing Hsp proteins encoded by the hsp genes. In O. oeni, CtsR is currently the only regulator of hsp genes. As an alternative to the lack of genetic tool, with the goal of understanding the mechanisms of O. oeni stress response, we developed a new expression vector, the pSIPSYN, to produce antisense RNA targeting of hsp18 mRNA. The synthesis of hsp18 asRNA leads to the decrease in the protein level of Lo18 and induced a loss of cultivability after heat or acid shock showing for the first time in vivo involvement of Lo18 in thermotolerance and a…
Molecular mechanisms involved in the regulation of stress response in Oenococcus oeni and experimental evolution
2020
Oenococcus oeni is the main bacterium responsible of malolactic fermentation in wine. This lactic acid bacteria grow in the stressful wine environment (high ethanol content, sulfites, low pH and low temperatures….). To maintain its cellular homeostasis, O. oeni has established mechanisms of resistance to its ecological niche. This research work focuses on the adaptative response of O. oeni to its environment and especially to acidity. Two approaches have been implemented (1) First, a targeted approach to characterize the molecular actors involved in regulating the general stress response. The RNA interference technique made possible the characterization of the repressor CtsR as well as two …